Evaluation of cell surface hydrophobicity and biofilm formation as pathogenic determinants among ESBL producing uropathogenic Escherichia coli


Original Article

Author Details : Amaresh Nigudgi, Vinay Hajare, Sunil Biradar, Anandkumar H*

Volume : 8, Issue : 4, Year : 2021

Article Page : 263-267

https://doi.org/10.18231/j.ijmr.2021.054



Suggest article by email


Abstract

Background: The attachment of the bacteria to the host cell and ability to invade the cell are regarded as important steps in the infectious process. The hydrophobicity of the microbial surface plays a critical role in the adherence of bacteria to the surface. The ability of biofilm formation can increase survival chance of microorganism, as cell growing in biofilm are highly resistant to the components of the immune system and many antimicrobial agents. Infection caused by ESBL- producers are associated with severe adverse outcomes and may be related to increased virulence of these strains.
Materials and Methods: A total of 100 urinary E.coli were selected for the study, of which 50 strains were from ESBL producers and 50 from non- ESBL-producing uropathogenic E. coli (UPEC) strains. The urinary E. coli isolates that were resistant to at least one of the three indicator cephalosporins (cefotaxime, cefpodoxime and ceftazidime) were tested for ESBL production by quantitative E-strip method. All the 100 urinary Escherichia coli strains were tested for cell surface hydrophobicity (CSH) by salt aggregation method and Biofilm production by tissue culture plate method.
Results: Among ESBL producers, 19 (38%) were CSH positive and 34 (68%) were biofilm producers. However among non-ESBL producers, 05 (10%) were CSH positive and 12 (24%) were biofilm producers.
Statistically significant difference (<0> Conclusion: In the present study, it was found that the ESBL producing isolates had a higher ability to form biofilm and CSH; both of them are among the important virulence factors associated with cell surface adherence which is the first step in bacterial infection.

Keywords: Uropathogenic E.coli, Virulence factors, ESBL


How to cite : Nigudgi A, Hajare V, Biradar S, Anandkumar H, Evaluation of cell surface hydrophobicity and biofilm formation as pathogenic determinants among ESBL producing uropathogenic Escherichia coli. Indian J Microbiol Res 2021;8(4):263-267


Copyright © 2021 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (creativecommons.org)





Article History

Received : 29-11-2021

Accepted : 29-11-2021

Available online : 15-12-2021


View Article

PDF File   Full Text Article


Copyright permission

Get article permission for commercial use

Downlaod

PDF File   XML File   ePub File


Digital Object Identifier (DOI)

Article DOI

https://doi.org/10.18231/j.ijmr.2021.054


Article Metrics






Article Access statistics

Viewed: 203

PDF Downloaded: 114



Open Abstract (Increase article citation) Wiki in hindi