A study of biofilm production and antimicrobial susceptibility pattern among urinary isolates

Original Article

Author Details : Ashwini Rajmane, Pankaj A Joshi*, Vishakha Shikhare, Meena Ramteerthakar, Vanita Kulkarni

Volume : 8, Issue : 4, Year : 2021

Article Page : 268-273


Suggest article by email


Background and Objectives: Urinary tract infection (UTI) is the most commonly acquired bacterial infection. Bacterial biofilms play an important role in urinary tract infections and are responsible for persistent infections as well as higher antimicrobial resistance. The microbial biofilms pose a public health problem as the microorganisms in the biofilms are difficult to treat with antimicrobial agents. So the present study was undertaken with the aim to study biofilm production and antimicrobial susceptibility pattern of urinary isolates.
Materials and Methods: Aerobic bacterial isolates from urine samples submitted to microbiology laboratory for culture were included in the study. The isolates were tested for biofilm formation by Congo red agar method and Christensen tube method. Antimicrobial susceptibility tests were performed on these isolates by Kirby Bauer disk diffusion method as per CLSI guidelines. A total of 293 Gram negative bacilli and 59 Gram positive cocci were tested for biofilm production and antimicrobial susceptibility testing.
Results: Gram-negative organisms were predominant (83.24%) of all the isolates. Biofilm production was detected in 47% of the isolates. Pseudomonas aeruginosa (51.7%), were the most common biofilm producing Gram negative bacilli followed by Escherichia coli (44.32%). Amongst Gram positive cocci, Enterococcus faecalis (77.8%) was the most common biofilm producing organism. Biofilm producing urinary isolates displayed relatively less percentage of antimicrobial susceptibility than biofilm non
Conclusion: Biofilm forming isolates showed higher antimicrobial resistance as compared to biofilm non producer. Early detection of biofilm production in urinary isolates may aid clinicians in the treatment of urinary tract infections.

Keywords: Biofilm, Urinary isolates, Antimicrobial susceptibility.

How to cite : Rajmane A, Joshi P A, Shikhare V, Ramteerthakar M, Kulkarni V, A study of biofilm production and antimicrobial susceptibility pattern among urinary isolates. Indian J Microbiol Res 2021;8(4):268-273

Copyright © 2021 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (creativecommons.org)

Article History

Received : 29-09-2021

Accepted : 06-10-2021

Available online : 16-12-2021

View Article

PDF File   Full Text Article

Copyright permission

Get article permission for commercial use


PDF File   XML File   ePub File

Digital Object Identifier (DOI)

Article DOI


Article Metrics

Article Access statistics

Viewed: 193

PDF Downloaded: 74

Open Abstract (Increase article citation) Wiki in hindi