- Visibility 156 Views
- Downloads 43 Downloads
- Permissions
- DOI 10.18231/2394-5478.2018.0044
-
CrossMark
- Citation
Prevalence and Antibiotic susceptibility pattern among Klebsiella isolates from patients attending a tertiary care hospital
- Author Details:
-
Sharanya K
-
Sindhu Cugati
-
Chitralekha Saikumar
Introduction: Klebsiella is an important opportunistic pathogen that is found to be the causative of hospital acquired pneumonia, urinary tract infections, skin and soft tissue infections and bacteremia. It has developed resistance to most of the currently used antibiotics and even resistance to higher antibiotics like carbapenems is on the rise. Hospital acquired infections caused by multidrug resistant strains of Klebsiella species are associated with high rates of morbidity and mortality.
Aim: To assess the diversity and state of resistance of K. pneumoniae to antibiotics in a tertiary care centre.
Materials and Methods: Klebsiella strains were isolated from clinical samples received in microbiology laboratory in a tertiary care centre and were identified by standard biochemical techniques. 368 Klebsiella strains were included in the study. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method following CLSI guidelines. Quality control was performed by using standard strain E. coli ATCC 25922.
Results: 13.16% of strains isolated from clinical samples were found to be Klebsiella species. Out of 368 Klebsiella strains 42% were resistant to Ceftriaxone, 46% to Cotrimoxazole, 52% to Amoxycillin-clavulanicacid. Resistance to Meropenem & Piperacillin-Tazobactum was 10% & 12% respectively.
Conclusion: Detection of drug resistance mechanisms, formulation of a good antibiotic policy and a proper antibiotic stewardship program should be incorporated to combat emerging multidrug resistance which is a global threat.
Keywords:Multidrug resistance, Klebsiella, Kirby-Bauer disk diffusion method
References
- Sourav Chakraborty, Kaniz Mohsina, Palash Kumar Sarker, Md Zahangir Alam, M Ismail Abdul Karim, S M Abu Sayem: Prevalence, antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and Klebsiella oxytoca among hospitalized patients. Period biol, (2016);118(1):53–8.
- Shilpa K, Ruby Thomas and Allavarapu Ramyashree: Isolation and Antimicrobial sensitivity pattern of Klebsiella pneumoniae from sputum samples in a tertiary care hospital, Int J Biomed and Adv Res (2016);7(2):053-
- C. Manikandan and A. Amsath; Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from urine samples, Int. J. Curr. Microbiol. App. Sci (2013);2(8):330-7.
- Manjula N. G., Girish C. Math., Kavita Nagshetty, Shripad A. Patil, Subhashchandra M. Gaddad, Channappa T. Shivannavar ; Antibiotic Susceptibility Pattern of ESβL Producing Klebsiella pneumoniae Isolated from Urine Samples of Pregnant Women in Karnataka, J Clin and Diagn Res (2014);8(10):DC08-DC11.
- Sarathbabu R., Ramani TV, Bhaskara rao K and Supriya Panda. Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from sputum, urine and pus samples. IOSR J Pharm Biol Sci. (2012);1(2):4-9.
- Jain A, Mondal R. Prevalence & antimicrobial resistance pattern of extended spectrum beta-lactamase producing Klebsiella sp isolated from cases of neonatal septicaemia. Indian J Med Res. (2007);125(1):89-94.
- C. Manikandan and A. Amsath; Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from urine samples, Int. J. Curr. Microbiol. App. Sci (2013);2(8):330-7.
- Babini GS, Livermore DM. Antimicrobial resistance amongst Klebsiella sps collected from intensive care units in Southern and Western Europe in 1997-1998. J Antimicrob Chemother. (2000);45(2):183-87.
- Navaneeth BV. Extended-spectrum and AmpC β- lactamases in Escherichia coli and Klebsiella pneumoniae from a rural South Indian tertiary care hospital. Int J Antimicrob Agents. (2007);29:602-3.
- Kazım Sahin, Ayse Tekin, Sule Ozdas, Demet Akin, Hande Yapislar, Aziz Ramazan Dilek and Emine Sonmez; Evaluation of carbapenem resistance using phenotypic and genotypic techniques in Enterobacteriaceae isolates. Ann Clin Microbiol Antimicrob (2015);14:44.
- Priyadarshini M. Deodurg, Rajive Kumar Sureka, Ravi D. Mala; Prevalence and antibiogram of extended spectrum β- lactamase producing Klebsiella pneumoniae in a tertiary care hospital. J Sci and Innov Rese (2014);3 (2):155-9.
- Rana S, Siddesh SB,Vijayaraghavan R. Prevalence and antibiogram of extended spectrum β- lactamase producing Klebsiella pneumonia and Proteus mirabilis in clinical isolates. Indian J Microbiol Res (2016);3(2):180-4.
- Enrico Magliano, Vittorio Grazioli, Loredana Deflorio, Antonia Isabella Leuci, Roberto Mattina, Paolo Romano et al. Gender and Age-Dependent Etiology of Sharanya K et al. Prevalance and Antibiotic susceptibility pattern among Klebsiella isolates… Indian Journal of Microbiology Research, April-June, 2018;5(2):208-212 212 Community-Acquired Urinary Tract Infections. The Scien Wor J, (2012):349-597
- Susethira A R, Uma A. Prevalence of Klebsiella Bacteriuria and Antimicrobial Susceptibility in a Tertiary Care Hospital, Tiruchirapalli, India. Int J Pharma and Clin Res (2016);8(6): 538-42.
- E Aktas, N Yigit, H Yazgi and A Ayyildiz; Detection of Antimicrobial Resistance and Extended-spectrum β- Lactamase Production in Klebsiella Pneumoniae Strains from Infected Neonates. J Int Med Res (2002);30(4):445–
- Shatalov, A. Prevalence and Antibiotic Resistance Pattern of Escherichia coli and Klebsiella pneumoniae in Urine Tract Infections at the La Paz Medical Center, Malabo, Equatorial Guinea. J of Med Microbiol (2015);5:177-83.
- Dr. Ph. Henkhoneng Mate, Dr. Kh. Sulochana Devi,Dr. Ksh. Mamta Devi, Dr. San Damrolien, Dr Ngangom Lilavati Devi, P. Pratita Devi; Prevalence of Carbapenem Resistance among Gram-Negative Bacteria in a Tertiary Care Hospital in North-East India, IOSR J Den and Med Sci. (2014);13(12):56-60.
- Ryan S. Arnold, MD, Kerri A. Thom, MD, MS, Saarika Sharma, MD MA, Michael Phillips,MD, J. Kristie Johnson, PhD, and Daniel J. Morgan, MD; Emergence of Klebsiella pneumoniae Carbapenemase (KPC)-Producing Bacteria, South Med J. (2011);104(1):40–5.
- Dr. R. Sarathbabu, Dr. T. V. Ramani, Dr. K. Bhaskara rao, Dr. Supriya Panda; Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from sputum, urine and pus samples, IOSR Journal of Pharmacy and Biological Sciences. (2012);1(2):04-9.
How to Cite This Article
Vancouver
K S, Cugati S, Saikumar C. Prevalence and Antibiotic susceptibility pattern among Klebsiella isolates from patients attending a tertiary care hospital [Internet]. Indian J Microbiol Res. 2018 [cited 2025 Oct 07];5(2):208-212. Available from: https://doi.org/10.18231/2394-5478.2018.0044
APA
K, S., Cugati, S., Saikumar, C. (2018). Prevalence and Antibiotic susceptibility pattern among Klebsiella isolates from patients attending a tertiary care hospital. Indian J Microbiol Res, 5(2), 208-212. https://doi.org/10.18231/2394-5478.2018.0044
MLA
K, Sharanya, Cugati, Sindhu, Saikumar, Chitralekha. "Prevalence and Antibiotic susceptibility pattern among Klebsiella isolates from patients attending a tertiary care hospital." Indian J Microbiol Res, vol. 5, no. 2, 2018, pp. 208-212. https://doi.org/10.18231/2394-5478.2018.0044
Chicago
K, S., Cugati, S., Saikumar, C.. "Prevalence and Antibiotic susceptibility pattern among Klebsiella isolates from patients attending a tertiary care hospital." Indian J Microbiol Res 5, no. 2 (2018): 208-212. https://doi.org/10.18231/2394-5478.2018.0044